Acute radiation syndrome-related gene expression in irradiated peripheral blood cell populations

Purpose In a nuclear or radiological event, an early diagnostic tool is needed to distinguish the worried well from those individuals who may later develop life-threatenFing hematologic acute radiation syndrome. We examined contribution of peripheral blood's cell populations on radiation-induced gene expression (GE) changes.Materials and methods EDTA-whole-blood six healthy donors was X-irradiated with 0 4Gy T-lymphocytes, B-lymphocytes, NK-cells granulocytes were separated using immunomagnetic methods. GE in whole blood.Results The contributed total RNA amount ratio 11.6 for 1.2 B-cells, NK-cells, 1.0 granulocytes. To estimate per population, baseline (0Gy) fold-change relative unexposed considered each gene. T-lymphocytes (74.8%/80.5%) predominantly up-regulation observed FDXR/DDB2 B-lymphocytes (97.1%/83.8%) down-regulated POU2AF1/WNT3 similar effect blood measurements reflecting corresponding order magnitude.Conclusions T- down-regulation POU2AF1/WNT3. This study underlines use biodosimetry purposes prediction health effects.